Plant Cell 南农:(H2O2流实验体系)硫化氢参与调节ABA诱导气孔关闭

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小木虫»成果分享»Plant Cell 南农:(H2O2流实验体系)硫化氢参与调节ABA诱导气孔关闭
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Plant Cell 南农:(H2O2流实验体系)硫化氢参与调节ABA诱导气孔关闭

 楼主| xuyue_2017 发表于 2020-2-11 18:53  
查看: 109059|回复: 0|显示全部楼层 |阅读模式
NMT是基因功能的活体检测技术,已被103位诺贝尔奖得主所在单位,及北大、清华、中科院使用。

主题:硫化氢参与调节ABA诱导气孔关闭的分子机制
标题:Persulfidation-based Modification of Cysteine  Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic  Acid Signaling
影响因子:8.631
检测指标:H2O2流速
检测样品:拟南芥保卫细胞
H2O2流实验处理方法:
4周龄的拟南芥幼苗,10μM ABA / 100μM NaHS瞬时处理
H2O2流实验测试液成份:10 mM MES,10 mM KCl,pH 6.15
作者:南京农业大学谢彦杰、沈杰、张晶、周明健、周恒


中文摘要(谷歌机翻)

越来越多的证据表明,硫化氢(H2S)是一种气体信号分子,通过过硫化作用来调节多种细胞信号传导途径,这涉及特定半胱氨酸残基的翻译后修饰(PTM)以形成过硫化物。但是,这种基于氧化还原的重要PTM的机理在高等植物中仍然知之甚少。

因此,我们已经分析了蛋白质过硫化如何在植物脱落酸(ABA)反应过程中作为一种特定且可逆的信号转导机制起作用。在这里,我们显示ABA以氧化还原依赖性方式刺激Cys44和Cys205的L-半胱氨酸脱硫酶1(DES1)(一种重要的内源H2S酶)的过硫化作用。此外,可持续的H2S积累会驱动Cys825和Cys890上NADPH氧化酶呼吸爆发氧化酶同源蛋白D(RBOHD)的过硫化,从而增强其产生活性氧的能力。在生理上,S-过硫化诱导的RBOHD活性与ABA诱导的气孔关闭有关。

这些过程共同形成一个负反馈回路,可微调保卫细胞氧化还原稳态和ABA信号传导。这些发现不仅扩展了我们目前在保卫细胞ABA信号传导方面对H2S功能的认识,而且还证明了涉及特定的和可逆的基于氧化还原的PTM的快速信号整合机制的存在,该机制是响应不断变化的环境条件而发生的。

(B) ABA- and NaHS-induced net H2O2 influxes in  guard cells of rbohD and pCAB3:RBOHD rbohD plants. Leaves from  4-week-old plant were preincubated for 3 hr in opening buffer (10 mM  MES, pH 6.15, and 10 mM KCl) under light (120 μE m−2s−1) and washed in  MES buffer three times for 15 min each. The net H2O2 influxes were  observed after ABA (10 μM) or NaHS (100 μM) treatment during the  indicated times (n = 6)
英文摘要

Accumulating evidence suggests that hydrogen sulfide  (H2S) is a gaseous signaling molecule that regulates diverse cellular  signaling pathways through persulfidation, which involves the post  translational modification (PTM) of specific cysteine residues to form  persulfides. However, the mechanisms that underlie this important  redox-based PTM remain poorly understood in higher plants.

We have, therefore, analyzed how protein  persulfidation acts as a specific and reversible signaling mechanism  during the plant abscisic acid (ABA) response. Here we show that ABA  stimulates the persulfidation of L-cysteine desulfhydrase 1 (DES1), an  important endogenous H2S enzyme, at Cys44 and Cys205 in a  redox-dependent manner. Moreover, sustainable H2S accumulation drives  persulfidation of the NADPH oxidase respiratory burst oxidase homolog  protein D (RBOHD) at Cys825 and Cys890, which enhance its ability to  produce reactive oxygen species. Physiologically,  S-persulfidation-induced RBOHD activity is relevant to ABA-induced  stomatal closure.

Together, these processes form a negative feedback  loop that fine-tunes guard cell redox homeostasis and ABA signaling.  These findings not only expand our current knowledge of H2S function in  the context of guard cell ABA signaling, but also demonstrate the  presence of a rapid signal integration mechanism involving specific and  reversible redox-based PTMs that occur in response to changing  environmental conditions.

Plant Cell 南农:(H2O2流实验体系)硫化氢参与调节ABA诱导气孔关闭
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